| COLⅣ

Type IV collagen (COL4) is the main collagen component of the basement membrane. It is a network-forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. It holds important signaling potential as subdomains such as tumstatin are released when the protein is degraded by special proteases. Consequently, type IV collagen is both the most important structural collagen of the basement membrane and it entails key signaling potential, which is important for various physiological and pathological functions. The most well-studied mutations in type IV collagen cause Alport syndrome, a chronic kidney disease.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.56	1.71	1.19	0.79	0.46	0.3	0.23	0.08
Corrected OD	2.48	1.63	1.11	0.71	0.38	0.22	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human COLⅣ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human COLⅣ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108